Design and development of poly-L/D-lactide copolymer and barium titanate nanoparticle 3D composite scaffolds using breath figure method for tissue engineering applications

نویسندگان

چکیده

In tissue engineering, the scaffold topography influences adhesion, proliferation, and function of cells. Specifically, interconnected porosity is crucial for cell migration nutrient delivery in 3D scaffolds. The objective this study was to develop a porous composite musculoskeletal engineering applications by incorporating barium titanate nanoparticles (BTNPs) into poly-L/D-lactide copolymer (PLDLA) using breath figure method. fabrication utilised 96/04 PLDLA, dioleoyl phosphatidylethanolamine (DOPE), different types BTNPs, including uncoated Al2O3-coated SiO2-coated BTNPs. BTNPs were incorporated polymer scaffold, which subsequently analysed field emission scanning electron microscopy (FE-SEM). biocompatibility each tested ovine bone marrow stromal stem morphology, viability, proliferation evaluated FE-SEM, LIVE/DEAD staining, Prestoblue assay. Porous scaffolds successfully produced, it observed that incorporation increased average pore size from 1.6 μm 16.2 (PLDLA/BTNP). PLDLA/BTNP provided suitable cells migrate inside while pure PLDLA with their much smaller size, elongated on surface. To conclude, method used scaffold. use resulted an optimal maintaining honeycomb-like structure. biocompatible yielded promising structures future applications.

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ژورنال

عنوان ژورنال: Colloids and Surfaces B: Biointerfaces

سال: 2021

ISSN: ['0927-7765', '1873-4367']

DOI: https://doi.org/10.1016/j.colsurfb.2020.111530